Lund University

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ISBS2015 - Towards Novel Blood Transfusion Therapies


Sunday June 21st, 2015

16:00 - 18:00     

Registration in the University Building  


Welcome address (Leif Bülow)



Monday June 22nd, 2015


Production of red blood cells for transfusion medicine (Palaestra)



8:00 -                  

Registration and poster set-up

9:00 - 9:10                            

Opening message: Professor Gunilla Westergren-Thorsson (Dean of the Medical Faculty at Lund University), Johan Flygare and Kenichi Miharada

9:10 - 9:40         

Opening lecture: Martin L Olsson, MD, PhD, Lund University, Sweden

Transfusion Medicine in the 21st Century - Current Challenges and Changing Concepts



Session 1            

In vitro red blood cell production I (Palaestra)

Chair: Johan Flygare

9:40 - 10:30       

James Palis, MD, University of Rochester Medical Center, NY, USA

Developmental regulation of erythroid self-renewal

10:30 - 11:00     

Coffee break

11:00 - 11:50     

Luc Douay, MD PhD, Université Pierre et Marie Curie-Paris, France                           

Progress in in-vitro red cell generation - are we there yet

11:50 - 12:00     

Photo session

12:00 - 13:30

Lunch and poster session (Palaestra)

12:15 - 13:00

Round Table Discussion (University Building room 206)

Chairman: Bo Hedlund, Biomedical Frontiers Inc, Minneapolis, Minnesota

The Dilemmas of the Academic Entrepreneur - License the Technology to Big Pharma or Start a New Company?



Session 2            

In vitro red blood cell production II (Palaestra)

Chair: Johan Flygare

13:30 - 14:00     

Emile van den Akker, PhD, Sanquin Institute, The Netherlands       

Control of erythroid erythropoiesis by tissue resident like macrophages: Increasing erythroid yield in vitro 

14:00 - 14:30

Kenichi Miharada, PhD, Lund University, Sweden

Production of mature red blood cells from immortalized erythroid cell lines

14:30 - 15:00

Joanne Mountford, PhD, University of Glasgow, UK

Serum Free, Feeder Free Erythroid Differentiation of Human Pluripotent Stem Cells

15:00 - 15:30     

Coffee break



Session 3            

In vitro red blood cell production III (Palaestra)

Chair: Kenichi Miharada

15:30 - 16:00

Marieke von Lindern, PhD, Sanquin Institute, The Netherlands

The role of mRNA translation in erythropoiesis

16:00 - 16:30

Johan Flygare, MD PhD, Lund University, Sweden

Direct lineage reprogramming of murine fibroblasts to erythroid progenitor cells by defined factors

16:30 - 17:00

Ashley Toye, PhD, University of Bristol, UK

Towards mimicking bone marrow; 3D erythroid cultures using scaffolds

17:00 - 17:15                        

Adam Sidaway, University of Bristol, UK

Analysis of the transcriptome of erythroid cells to identify factors that promote proliferation

17:15 - 17:30     

Jan Frayne, PhD, University of Bristol, UK

The first human immortalised cell line generated from adult erythroid cells                   

17:30 - 17:45     

Marieangela Wilson, PhD, University of Bristol, UK

The development of methodology for proteome-wide profiling, and comparative analysis of transcription factors in erythroid cells

Free evening




Session 4

HBOCs from industrial laboratories, in parallel with Sessions 2 and 3 (AF Borgen)


Chairs: Thomas Chang and Leif Bülow

13:30 - 13:55

Gord Adamson, Therapure Biopharma Inc., Mississauga, Canada

Hemoglobin-Based Products for Drug Delivery and Organ Perfusion

13:55 - 14:20                        

Franck Zal, Hemarina SA, Morlaix, France

Optimisation of Mesenchymal Stem Cell functions and proliferation: Investigation of the benefits of a new oxygen carrier, HEMOXCell®, in platelet lysate-supplemented media

14:20 - 14:45     

Peter Keipert, Keipert Corp, San Diego, USA

Clinical evaluation of MP4OX as an oxygen therapeutic adjunct to acute resuscitation of trauma patients in severe hemorrhagic shock

14:45 - 15:10

Jan Simoni, Texas HemoBioTherapeutics & BioInnovation Center, Lubbock, USA

Current Status of Clinical Development of Hb-Based Oxygen Therapeutic with Pharmacologic Properties of ATP, Adenosine and Reduced Glutathione (GSH)                 

15:10 - 15:40

Coffee break

15:40 - 16:05      

Carleton Hsia, NanoBlood LLC, Sioux Falls, USA

The Second Quantum Revolution - nanoQuantum Medicine - The Introduction of nano Red Blood Cell (nanoRBC) for Use in Critical and Chronic Care Medicine

16:05 - 16:30

Kim D. Vandegriff,  NovoSang, Inc., San Diego, CA, USA

Hemoglobin extravasation in the brain of rats exchange-transfused with hemoglobin-based oxygen carriers         

16:30 - 16:55

Abraham Abuchowski, Prolong Pharmaceuticals. South Plainfield, USA                  

Sanguinate™: A CO/O2 delivery therapeutic for the treatment of anemic and ischemic disorders

16:55 - 17:20  

Jonathan S Jahr, Anesthesiology and Perioperative Medicine, David Geffen School of Medicine at UCLA, USA

Post Marketing Analysis of Safety and Efficacy of  Hemoglobin Glutamer-250 (Bovine), [HEMOPURE®, HBOC-201)] in South Africa, On Behalf of the HEMOPURE South African Task Force

17:20 -                  

Abdu I Alayash, Center for Biologics Evaluation and Research, Food and Drug Administration, USA

Tribute - Dr. Joseph Fratantoni

Free evening






Tuesday June 23rd, 2015



Hemoglobin Production, Engineering and HBOC Design (Palaestra)


Session 5   

Fundamental understanding of hemoglobin

Chairs: Michael Wilson and Chengmin Yang



8:00 -  Registration

8:30 - 9:00

Abdu I Alayash, Center for Biologics Evaluation and Research, Food and Drug Administration, USA

Oxidative Pathways in Hemoglobin: Do They Really Matter?

9:00 - 9:30

John S Olson, Rice University, USA      

Protein Engineering Strategies for Recombinant Hb-Based Oxygen  Carriers(rHBOCs): Compromises between Hemoglobin Stability, Production, Function, and Toxicity In Vivo

9:30 - 9:45

José Luis Martinez, Chalmers, Sweden  

Production of recombinant human hemoglobin by microbial fermentation through yeast metabolic engineering

9:45 - 10:00 

Khuanpiroon Ratanasopa, Lund University, Sweden

Genetically Linked Human fetal Hemoglobin: Strategy to enhance recombinant hemoglobin production and produce HBOCs

10:00 - 10:30     

Coffee break



Session 6

HBOC design

Chairs: Ken W Olsen and Peter Keipert



10:30 - 11:00

Chengmin Yang, Institute of Blood Transfusion, CAMS & PUM, PR China

Some Thoughts on Research and Development of HBOCs in China

11:00 - 11:30

Hiromi Sakai, Nara Medical University, Japan

Translational Research of Hemoglobin-vesicles as a Transfusion Alternative                             

11:30 - 12:00

Lian Zhao, Transfusion Medicine, Academy of Military Medical Sciences, Beijing, PR China

Construction of blood-compatible hemoglobin-loaded nanoparticles as oxygen carriers for in vivo oxygenation                   

 12:00 - 12:15                          

Ka Zhang, Lund University, Sweden

Characterization of human hemoglobin-imprinted polymer beads – recognition and protection against lipid peroxidation

12:15 - 13:10  

Lunch and poster session (Palaestra)

12:20 - 13:05

Round Table Discussion (University Building room 206)

Chairman: Bo Hedlund, Biomedical Frontiers Inc, Minneapolis, Minnesota

The Dilemmas of the Academic Entrepreneur - License the Technology to Big Pharma or Start a New Company?



Session 7             

HBOC engineering I

Chairs: Chris Cooper and Lian Zhao



13:15 - 13:45

Thomas MS Chang, McGill University, Montreal, Canada               

Red Blood Cell replacement or nanobiotherapeutics with enhanced red blood cell functions?

13:45 - 14:10

Hae Won Kim, Brown University, Providence, USA

A new collaboration model for facilitated HBOC development            

14:10 - 14:35  

Jiaxin Liu, Blood Transfusion, CAMC, Chengdu, P. R. China

Resuscitation with polymerized human placenta hemoglobin improves tissue oxygenation and survival in a rat hemorrhagic shock model                        

14:35 - 15:00          

Hans Bäumler, Transfusion Medicine, Charité-Universitätsmedizin Berlin, Germany


Novel Hemoglobin Particles—Promising New-Generation Hemoglobin-Based Oxygen Carriers 

15:00 - 15:30

Coffee break      



Session 8                           

HBOC engineering II

Chairs: Hiromi Sakai and Hans Bäumler



15:30 - 15:55                            

Ken W Olsen, Loyola University Chicago, USA

Development of Inside-Out PEGylated Crosslinked Hemoglobin Polymers

15:55 - 16:10        

Sandeep Chakane, Lund University, Sweden

A recombinant unstructured polypeptide to increase stability of fusion HbF: an alternative to PEGylation

16:10 - 16:30                           

Chanin Nantasenamat, Mahidol University, Bangkok, Thailand

Predicting the oxygen affinity of human hemoglobin

16:30 - 16:50       

YanJon Guo, National Center for Nanoscience and Technology, Beijing PR China

Application of Raman spectroscopy in the structure and function study of hemoglobin                        

17:15 - 18:15

Organ concert - Lund Cathedral


Buses for conference dinner leaving outside Lund Cathedral                                

19:15 - 22:00

Conference dinner at Kulturens Östarp               



Wednesday June 24th, 2015      



Extracellular hemoglobin and Protection Proteins (Palaestra)



Session 9            

Hemoglobin toxicity and protection

Chairs: Abdu Alayash and Brandon Reeder



8:00 -          


8:30 - 9:00  

Magnus Gram, Skåne University Hospital, Lund, Sweden

Pathophysiology of extracellular hemoglobin: From molecular mechanisms to clinical significance

9:00 - 9:30

Dominik Schaer, Zürich, Switzerland

Protection against cell free hemoglobin toxicity by haptoglobin

9:30 - 10:00

Jozsef Balla, University of Debrecen, Debrecen, Hungary

Heme, heme oxygenase, and ferritin: how the vascular endothelium and smooth muscle cells survive (and die) in an iron-rich environment

10:00 - 10:30                             

Coffee break

10:30 - 11:00      

Ann Smith, University of Missouri, Kansas City, USA

Mechanisms of heme toxicity and hemopexin as a therapeutic                                             

11:00 - 11:30

Sören Moestrup, Aarhus University, Aarhus, Denmark

Receptor systems for hemoglobin and heme – role in inflammation                                                       

11:30 - 12:00                        

Isaac K Quaye, University of Namibia School of Medicine, Windhoek, Namibia

Plasticity in macrophage function: A pathway for intervention in hemoglobin release?

12:00 - 13:00  

Lunch and poster session (Palaestra)

13:00 - 13:30      

Bo Åkerström, Lund University, Lund, Sweden

A1M – an extravascular heme-binding and tissue cleaning protein with therapeutic potential

13:30 - 14:00

Chris Cooper, Essex University, Essex, UK

Modification of Tyrosine Electron Transfer Pathways in Hemoglobin decreases oxidative reactivity

14:00 - 14:30

Willem A Buurman, Maastricht University, Netherlands

Hemolysis during cardiac surgery

14:30 - 14:50    

Leif Bülow, Lund University, Sweden

From the white buses to the white trucks - or how to convert HBOCs into viable products                                        

14:50 - 15:00 Closing remarks

15:00 - 15:10  

Thomas MS Chang, McGill University, Montreal, Canada






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University Building

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